Smac-N7 Peptide; Cell-Permeable

Biochem/physiol Actions

Primary TargetPromotes procaspase-3 activation

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

The Smac-N7 peptide (Cat. No. 567370) is modified to be cell permeant by linking the lysine carboxyl terminal to the arginine of Antennapedia homeodomain 16-mer peptide via a proline linker.

The Smac-N7 peptide (Cat. No. 567370) where the lysine carboxy terminal is linked to the arginine of Antennapedia homeodomain 16-mer peptide (underlined) via a proline linker to make it cell-permeable. Smac-N7 peptide contains the N-terminal seven residues of Smac and promotes the activation of procaspase-3 (~10 µM). Also, promotes the catalytic activity of caspase-9 in the presence of XIAP.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Srinivasula, S.M., et al. 2001. Nature410, 112.Chai, J., et al. 2000. Nature406, 855.Liu, Z., et al. 2000. Nature408, 1004.Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-Pro-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-OH

Warning

Toxicity: Standard Handling (A)